NanoTemper Technologies

Binding Affinity and Protein Stability

Affinity. Stability. Conformation.

NanoTemper Technologies, headquartered in Munich, Germany, is a leading provider of highly innovative instrumentation and software solutions for biomolecular analytics. We are driven by the unique demands of science and committed to developing high-quality instruments that push the limits of speed, efficiency and precision. Our proprietary technologies are used by pharmaceutical and basic research scientists across four continents. We back our products with unparalleled customer care through a team of experts at our global application support centers. NanoTemper Technologies has received the German Innovation Award (2012), the Bavarian Export Prize (2013) and the Deutscher Gründerpreis (German Founders Award; 2014).

Monolith: More than binding affinities

The Monolith NT.115 uses MicroScale Thermophoresis (MST) to quantify biomolecular interactions and calculate dissociation constants (Kd) in an easy, rapid and accurate way. During an MST experiment, molecules move along a microscopic laser-induced temperature gradient. Upon binding, the molecule of interest changes its migration behavior, which is detected using covalent dyes, fluorescent fusion proteins or intrinsic fluorescence (Monolith NT.LabelFree). By combining the precision of fluorescence detection with the flexibility and sensitivity of thermophoresis, Monolith instruments provide a flexible, robust and highly versatile platform for measuring molecular interactions. The fully-automated Monolith NT.Automated is designed for easy handling and high-throughput applications. The instrument can be integrated in a liquid handling platform to allow several hours of unsupervised data acquisition. The capillary chips are easy to use and avoid any equilibration or washing downtime. Thus, the Monolith NT.Automated is ideal for screening and hit identification applications.

Prometheus: Rapid & easy protein stability analysis

Prometheus NT.48 is based on nanoDSF, an advanced Differential Scanning Fluorimetry technology that uses intrinsic tryptophan and tyrosine fluorescence to measure protein folding and stability in a label-free manner. The capillary format eliminates instrument maintenance requirements and enables precise measurements and easy sample handling, even for highly viscous formulation conditions or detergent-solubilized membrane proteins. The instrument provides unmatched scanning speed and data point density, revealing even small unfolding events. Prometheus instruments utilize an inverse light scattering technology to combine thermal stability analysis with determination of aggregation onset temperatures. Together with the NT.Robotic Autosampler, the Prometheus NT.Plex can be fully automated. This setup allows for unattended sample preparation, handling and measurement, and is amenable to virtually an unlimited number of samples.

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+49 89 4522895 0

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NanoTemper Technologies
Floessergasse 4
81369, Munich
Germany

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